The short answer: Thymoquinone (TQ) is a natural compound found in the oil of Nigella sativa seeds, and it is the figure most brands use to describe their oil. "Min 2% TQ" on our label means each batch is tested and contains at least 2% thymoquinone by weight. Origin, cold pressing and storage all influence that number.

Thymoquinone is a small aromatic molecule (a quinone, formula C10H12O2) that occurs naturally in black seed and gives the oil part of its sharp, peppery aroma. It sits in the oil's volatile fraction, alongside related compounds such as p-cymene, and it is the constituent researchers name most often when describing Nigella sativa oil.
Labs usually measure thymoquinone with liquid chromatography (HPLC), which separates the oil's components and quantifies each one against a reference standard. The result is reported as a percentage of the oil by weight, or in milligrams per gram.
2% by weight means at least 20 mg of thymoquinone per gram of oil. Our liquid label serving is 1 teaspoon (5 g), so one serving contains at least about 100 mg of TQ as a constituent of the oil. Our 2000 mg softgel serving (2 softgels) works out to at least about 40 mg.
| Product | Label serving | Oil per serving | TQ at Min 2% |
|---|---|---|---|
| Liquid black seed oil | 1 tsp | 5 g | ≥ 100 mg |
| Softgels 2000 mg | 2 softgels | 2,000 mg | ≥ 40 mg |
| Softgels 1000 mg | 2 softgels | 1,000 mg | ≥ 20 mg (standardized at 2%) |
Not necessarily. Very high figures sometimes come from oils that have been blended with isolated thymoquinone or essential-oil fractions, which changes the product from a whole cold-pressed seed oil into something else. We prefer a whole, unrefined oil from one origin with a tested, honest minimum.
Ask for a recent certificate of analysis showing the method (HPLC) and the batch number, and make sure the percentage is printed on the bottle rather than only in an ad.
Is thymoquinone the same as black seed oil?
No. Thymoquinone is one natural compound inside black seed oil, which also contains fatty acids such as linoleic and oleic acid.
Does refrigeration matter for TQ?
Refrigeration is not required. A cool, dark, dry cupboard and a tightly closed amber bottle are enough.
Do your softgels contain the same oil?
Yes. Our softgels contain cold-pressed Nigella sativa oil from the same single origin in Turkey, in a gelatin softgel.

Lifestyle photo. Not the reviewer.
★★★★★
“I love the Black seed oil capsules. I would highly recommend them to anyone.”
Shannon L.
Review of Black Seed Oil Softgels 2000 mg, mothernatureorganics.com

Lifestyle photo. Not the reviewer.
★★★★★
“Mother Nature's black seed oil is the very best quality. The price is reasonable as well.”
Edward P.
Review of Black Seed Oil Softgels 2000 mg, mothernatureorganics.com
★★★★★
“Love it this is the only brand I'll buy.”
Reggie W.
Review of Organic Black Seed Oil, mothernatureorganics.com
Shop black seed oil with a tested TQ minimum
Mother Nature Organics, founded 2016. Every order is backed by our 60-day money-back guarantee.
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
You're $49.00 away from free shipping